Enzyme Immunoassay for Serum 18-Hydroxycorticosterone and Its Clinical Application
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چکیده
منابع مشابه
Enzyme immunoassay of urinary mevalonic acid and its clinical application.
We have developed an enzyme immunoassay for mevalonic acid (MVA), using a specific monoclonal antibody. The intra- and interassay coefficients of variation calculated on two urine samples were 3.3% and 3.4%, respectively, in the intraassay precision test and 3.5% and 6.9% in the interassay evaluation. Pravastatin, a 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitor, was admini...
متن کاملEnzyme immunoassay for autoantibodies to human liver-type arginase and its clinical application.
BACKGROUND Arginase is an enzyme of the urea cycle, and one of the two isoenzymes is the liver-type enzyme. We examined serum autoantibodies to this liver-type enzyme in patients with hepatitis. METHODS Antibodies to recombinant human liver-type arginase were measured by ELISA in 95 patients and 55 healthy controls. RESULTS The mean absorbance values in the ELISA assays of patients with def...
متن کاملAn enzyme immunoassay for galactosyltransferase isoenzyme II, and its clinical application to cancer diagnosis.
In this "sandwich"-type enzyme immunoassay of galactosyltransferase isoenzyme II (GT-II), we used the monoclonal antibody MAb 3872 previously established and characterized to be specific for GT-II. Plastic beads coated with MAb 3872 were incubated with serum and buffer, washed, then incubated with MAb 3872 conjugated with horseradish peroxidase and again washed. Peroxidase activity remaining on...
متن کاملSolid-phase enzyme immunoassay for serum ferritin.
We describe a solid-phase enzyme immunoassay for ferritin in human serum, with use of a horseradish peroxidase-labeled antibody and a highly sensitive chromogen, 2,2'-azino-di(3-ethyl-benzthiazoline-6-sulfonate). The assay requires only 10 microL of serum per assay, relatively less time and labor than other assys for ferritin, and as little as 10 pg of ferritin can be measured. We believe this ...
متن کاملHomogeneous enzyme immunoassay for triiodothyronine in serum.
BACKGROUND The concentration of triiodothyronine (T3) in human serum is extremely low and can be determined only by very sensitive methods. We developed a homogeneous enzyme immunoassay for T3 analysis in unextracted serum. METHODS A T(3) derivative was conjugated to the -SH groups of glycogen phosphorylase b (GPb) from rabbit muscle. Conjugation caused inhibition of enzyme activity, and the ...
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ژورنال
عنوان ژورنال: Folia Endocrinologica Japonica
سال: 1985
ISSN: 0029-0661
DOI: 10.1507/endocrine1927.61.10_1157